We report an optical dipole trap of strontium monohydroxide (SrOH) with 1400(300) trapped molecules. Through optical pumping, we access vibrational states that are proposed for improved probes of the electron’s electric dipole moment (eEDM) and ultralight dark matter (UDM). For each of these states, the lifetime of trapped molecules is measured, and found to be consistent with spontaneous radiative decay and black-body excitation limits, making this platform viable for these eEDM and UDM searches.